DCA and CancerDCA as a Cancer Treatment - Sodium Dichloroacetate

Docosahexaenoic Acid and Butyrate Synergistically Induce Colonocyte Apoptosis by Enhancing Mitochondrial Ca2+ Accumulation

Satya Sree N. Kolar1, Rola Barhoumi2,3, Joanne R. Lupton1,2,3 and Robert S. Chapkin1,2,3
1 Faculty of Nutrition, 2 Department of Veterinary Integrative Biosciences, and 3 Center for Environmental and Rural Health, Texas A&M University, College Station, Texas

Requests for reprints: Robert S. Chapkin, Department of Nutrition and Food Science, Kleberg Biotechnology Center, MS 2253, Texas A&M University, College Station, TX 77843-2253. Phone: 979-845-0448; Fax: 979-862-2378; E-mail: r-chapkin@tamu.edu.

We have previously shown that butyrate, a short-chain fatty acid fiber fermentation product, induces colonocyte apoptosis via a nonmitochondrial, Fas-mediated, extrinsic pathway. Interestingly, fermentable fiber when combined with fish oil containing docosahexaenoic acid (DHA, 22:6n-3) exhibits an enhanced ability to induce apoptosis and protect against colon tumorigenesis.

To determine the molecular mechanism of action, the effect of DHA and butyrate cotreatment on intracellular Ca2+ homeostasis was examined. Mouse colonocytes were treated with 50 µmol/L DHA or linoleic acid (LA) for 72 h ± butyrate (0–10 mmol/L) for the final 24 h. Cytosolic and mitochondrial Ca2+ levels were measured using Fluo-4 and Rhod-2. DHA did not alter basal Ca2+ or the intracellular inositol trisphosphate (IP3) pool after 6 h butyrate cotreatment. In contrast, at 12 and 24 h, DHA- and butyrate-treated cultures exhibited a 25% and 38% decrease in cytosolic Ca2+ compared with LA and butyrate. Chelation of extracellular Ca2+ abolished the effect of thapsigargin on the IP3-releasable Ca2+ pool. DHA and butyrate cotreatment compared with untreated cells increased the mitochondrial-to-cytosolic Ca2+ ratio at 6, 12, and 24 h by 73%, 18%, and 37%, respectively.

The accumulation of mitochondrial Ca2+ preceded the onset of apoptosis. RU-360, a mitochondrial-uniporter inhibitor, abrogated mitochondrial Ca2+ accumulation and also partially blocked apoptosis in DHA and butyrate cotreated cells. Collectively, these data show that the combination of DHA and butyrate, compared with butyrate alone, further enhances apoptosis by additionally recruiting a Ca2+-mediated intrinsic mitochondrial pathway. [Cancer Res 2007;67(11):5561–8]


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